Journal: eNeuro

Article Title: Essential Role of Somatic Kv2 Channels in High-Frequency Firing in Cartwheel Cells of the Dorsal Cochlear Nucleus

doi: 10.1523/ENEURO.0515-20.2021

Figure Lengend Snippet: Antibody information

Article Snippet: Kv2.1 (L80/21) , Synthetic peptide 837–853 aa of rat Kv2.1 , Mouse IgG3 mAb , NeuroMab catalogue 75-315, RRID: AB_2315863 , 1 μg/ml.

Techniques: Concentration Assay, Purification

Journal: Cardiovascular Therapeutics

Article Title: Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b

doi: 10.1155/2022/7145699

Figure Lengend Snippet: qRT-PCR primers for the HEK293 cells Kv channel mRNA and actin.

Article Snippet: After blocking, the membranes were probed with primary antibodies against Kv2.1 (19963-1-AP, NM_004975, Proteintech) and β -actin (TA-09, Zhong Shan Jin Qiao Biotechnology, Shanghai, China) followed by incubation with goat anti-rabbit (7074S, Cell Signaling Technology) and mouse (SA00001-1, Proteintech) IgG, horseradish peroxidase- (HRP-) linked secondary antibody, as required. β -Actin was used as an internal control.

Techniques:

Journal: Cardiovascular Therapeutics

Article Title: Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b

doi: 10.1155/2022/7145699

Figure Lengend Snippet: qRT-PCR primers for mouse Kv channel mRNA and actin.

Article Snippet: After blocking, the membranes were probed with primary antibodies against Kv2.1 (19963-1-AP, NM_004975, Proteintech) and β -actin (TA-09, Zhong Shan Jin Qiao Biotechnology, Shanghai, China) followed by incubation with goat anti-rabbit (7074S, Cell Signaling Technology) and mouse (SA00001-1, Proteintech) IgG, horseradish peroxidase- (HRP-) linked secondary antibody, as required. β -Actin was used as an internal control.

Techniques:

Journal: The European Journal of Neuroscience

Article Title: Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells

doi: 10.1111/ejn.12526

Figure Lengend Snippet: Antibodies

Article Snippet: Kv2.1 , Mouse , aa. 211–229 of rat Kv2.1 , 3.6 , , NeuroMab , 75–159 , Lim et al . ( ) .

Techniques:

Journal: Scientific Reports

Article Title: Effects of Trans, Trans-2,4-decadienal on the Ions Currents of Cardiomyocytes: Possible Mechanisms of Arrhythmogenesis Induced by Cooking-oil Fumes

doi: 10.1038/s41598-020-62733-1

Figure Lengend Snippet: Expression of Kv2.1 ( a ) and Cav1.2 ( b ) measured by western blotting in the H9c2 cells after treatment with tt-DDE-free media for 18 hours (control group), tt-DDE-containing media for 18 hours (1 µM; tt-DDE group). Compared with the control group, the expression of Kv2.1 was significantly decreased in the tt-DDE group. In contrast, expression of Cav1.2 did not significantly differ between the two groups. Data are mean ± standard deviation; *P < 0.05 compared with the control.

Article Snippet: The proteins were transferred to Immobilon PVDF membranes (Merck Millipore, Darmstadt, Germany) and incubated with rabbit polyclonal antibodies for Kv2.1 (Merck Millipore, Darmstadt, Germany) and Cav1.2 (Sigma-Aldrich, Darmstadt, Germany) for 1 hour at room temperature.

Techniques: Expressing, Western Blot, Standard Deviation